Effect of hypoxia on production of caprine chimeric blastocyst

This study was conducted to evaluate the effect of 5% and 21% oxygen concentrations on chimeric embryo development. Inner cell mass (ICM) from parthenogenetic activated blastocyst were used produce ES cell-like cells. A pair zona-free tetraploid embryos one clump pESCs at passage 2 3 aggregated granulosa monolayer in such a manner that sandwiched between embryos. The aggregates prepared randomly divided into two groups viz. Group 1 (21% O2) (n=54) (5% (n=50) followed by culture humidified atmosphere CO2 38.5°C incubator. Aggregation rate quality similar both groups. percentage aggregation, 8-16 cell, morula 91.66±4.04%, 49.07±5.08%, 25.92±6.05% 16.66±4.95%, respectively while 96.59±2.83%, 51.89±9.14%, 16.28±5.52% 28.40±8.10%, respectively. stage formed comparatively higher than formation lower O2). Also, had significantly more blastocysts as compared To conclude, under O2 leads improved aggregation also enhances production rather with atmospheric (21%) during incubation.